The IDO1 Cellular Activity QuickDetectTM Supplements are designed tocomplement the hIDO1-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO1-expressing cell lines. This kit contains the cell culture medium supplements necessary foractivation of IDO1 and for the analysis and detection of tryptophan 2,3 dioxygenase (IDO1)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and thedetection reagents, when used as described, allow for indirect measurement of Kyn levels byanalyzing absorption at 480 nm.