PRMT4 Chemiluminescent Assay Kit

The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylatedPRMT4 substrate. With this kit, only three simple steps on a microtiter plate are requiredfor methyltransferase detection. First, S-adenosylmethionine is incubated with a samplecontaining assay buffer and methyltransferase enzyme. Next, primary antibody is added.Finally, the strip plates are treated with an HRP-labeled secondary antibody followed byaddition of an HRP substrate to produce chemiluminescence that can then be measuredusing a chemiluminescence reader.