PRMT3 Chemiluminescent Assay Kit

The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highlyspecific antibody that recognizes methylated substrate. With this kit, only three simplesteps on a microtiter plate are required for methyltransferase detection. First,Sadenosylmethionine is incubated with a sample containing assay buffer andmethyltransferase enzyme for one hour. Next, primary antibody is added. Finally, theplate is treated with an HRP-labeled secondary antibody followed by addition of the HRPsubstrate to produce chemiluminescence that can then be measured using achemiluminescence reader.