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Affinity purified Monoclonal Anti-V5-Tag, clone E10/V4RR, mouse IgG1. Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins. Affinity purified from culture supernatant of hybridoma cells. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA (1:5000-10000), Immunocytochemistry (1:500-2000), Immunoprecipitation , Frozen sections (1:500-2000), Paraffin sections. Optimal dilutions/concentrations should be determined by the end user.