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The Activated Protein C (APC, also known as PROC) Assay Kit is a 96-well format fluorogenic assay designed to measure the protease activity of Activated Protein C (PROC) for screening and profiling applications.
Illustration of the assay principle.
The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent AMC. Fluorescence intensity increases proportionally to the activity of the protease.