PDE4C Assay Kit

The PDE4C1 Assay Kit is designed for identification of PDE4C1 inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4C1 to the binding agent. The key to the PDE4C1 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4C1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4C1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.