TNKS2 Homogenous Assay Kit

The TNKS2 (also known as PARP5b) Homogeneous Assay Kit is designed to measure TNKS2 activity for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to its substrate. The key to the TNKS2 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for TNKS2 reactions. First, a sample containing TNKS2 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Figure 1: TNKS2 (PARP5b) Homogenous Assay Kit schematic