TNKS1 Homogenous Assay Kit

The TNKS1 (also known as PARP5a) Homogeneous Assay Kit is designed to measure TNKS1 activity for screening and profiling applications. TNKS1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to its substrate. The key to the TNKS1 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, a sample containing TNKS1 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Figure 1: TNKS1 (PARP5a) Homogenous Assay Kit schematic