TNKS1 (PARP5A) Colorimetric Assay Kit

The TNKS1 Colorimetric Assay Kit is designed to measure the activity of Tankyrase 1 (TNKS1, also known as PARP5A) for screening and profiling applications. TNKS1 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The TNKS1 assay kit comes in a convenient 96-well format, with purified TNKS1 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions. The key to the TNKS1 (PARP5A) Colorimetric Assay Kit is the biotinylated NAD+. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can be measured using a UV/Vis spectrophotometer microplate reader.

Figure 1. TNKS1 (PARP5A) Colorimetric Assay Kit schematic

*NOTE: As of March 2023, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request