EZH2 Homogeneous Assay Kit

The EZH2 Homogeneous Assay Kit is designed to measure EZH2 activity for screening and profiling applications. EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27. The key to the EZH2 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing EZH2 enzyme is incubated with the histone substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.