EHMT1 (GLP) Chemiluminescent Assay Kit

The EHMT1 (GLP) Chemiluminescent Assay Kit is designed to measure EHMT1 (also known as G9a-like protein) activity for screening and profiling applications. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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