C-CBL-Driven Tyro3 Ubiquitination Intrachain TR-FRET Assay Kit

●Purchase●DescriptionTheC-CBL-drivenTyro3UbiquitinationIntrachainTR-FRETAssayKitisasensitivehigh-throughputscreening(HTS)TR-FRETAssayKit

产品型号：78408

更新时间：2024-11-28

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详细介绍

品牌	BPS Bioscience

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Description

The C-CBL-driven Tyro3 Ubiquitination Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure C-CBL E3 ligase activity in a homogeneous 384 reaction format. It utilizes a Europium cryptate-labeled Ub (donor) and a Cy5-labeled Ub (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput applications as well as real-time analyses of polyubiquitination. Of note, the assay kit does not detect mono-ubiquitination.

Alternatively, under the same experimental conditions the AXL kinase (BPS Bioscience #40180) can be used instead of Tyro3 as C-CBL ubiquitination substrate.

78408 TR-FRET Schematic

Figure 1. C-CBL-driven Tyro3 ubiquitination intrachain TR-FRET Assay Kit schematic

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Synonyms

Tyrosine-protein kinase receptor, TYRO3, BYK, DTK, RSE, SKY, TIF, CBL2, RNF55, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase CBL

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