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Complex of human recombinant PRMT and MEP50 proteins. Recombinant human PRMT5 (protein arginine methyltransferase 5, variant 1), encompassing amino acids 2-637(end). This construct contains an N-terminal Flag-tag. Recombinant human MEP50 (WD repeat domain 77, variant 2), encompassing amino acids 2-342(end). This construct contains an N-terminal His-tag (6xHis). The two proteins were co-expressed and affinity purified.
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