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Recombinant human JAK2 (Janus Kinase 2) encompassing amino acids 536-812, containing the pseudo-kinase domain JH2 with mutations W659A, W777A and F794H. This construct contains a C-terminal His-tag (6xHis) followed by an Avi-Tag™. The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.