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Recombinant human KRAS (GTPase), isoform b, encompassing amino acids 2-185(end). This construct contains an N-terminal His-tag (6xHis) mutation of interest G12V. KRAS (G12V) His-Tag (BPS Bioscience #100480) was affinity purified and was loaded with BODIPY-GDP. Unbound BODIPY-GDP was removed by spin column and the protein is ready for a GDP/GTP exchange assay.