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Recombinant human PCSK9 (Proprotein convertase subtilisin/kexin type 9), encompassing amino acids 31-692 (end). The protein contains mutation of interest D374T, and a C-terminal His-tag (6xHis) followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.